Abstract
The purpose of this study is to identify and isolate fungus from motichoor ladoos from the Hadapsar region in Pune area of Maharashtra, India. Sequence analysis, BLAST searches, PCR amplification, sequencing of the internal transcribe spacer region (ITS) were used to characterize the isolated fungi. After BLAST analysis, the obtained sequences were compared to the sequences from GenBank, the corresponding fungal isolate was identified at the species level (Alternaria burnsii).